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CAS 7365-45-9 HEPES Molecular Biology Grade Free Acid 99.5%

Categories Biochemical Reagents
Brand Name: YunbangPharm
Model Number: YB015
Certification: ISO9001
Place of Origin: China
MOQ: Negotiable
Price: Negotiable
Payment Terms: L/C, D/A, D/P, T/T, Western Union
Supply Ability: 200kg
Delivery Time: 5-8 working days
Packaging Details: 1kg/Bottle, 5-25kg/Drum
Cas No: 7365-45-9
Application: Biochemical Reagents
Purity: 99%
Classification: Biological Buffer
Form: crystal powder
Color: White
Samples: Available
Package: 1kg/5kg/25kg
Transport: Courier/Air/Sea
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CAS 7365-45-9 HEPES Molecular Biology Grade Free Acid 99.5%

CAS 7365-45-9 HEPES Molecular Biology Grade (Free Acid)

HEPES Basic information
Product Name:HEPES
Mol File:7365-45-9.mol
HEPES Structure
HEPES Chemical Properties
Melting point234-238 °C
density1.07 g/mL at 20 °C
refractive indexn20/D 1.339
storage temp.2-8°C
solubilityH2O: 1 M at 20 °C, clear, colorless
PH5.0-6.5 (1Maqueous solution)
PH Range7.0 - 7.6
pka7.5(at 25℃)
Water SolubilitySoluble
λmaxλ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Stability:Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
HEPES Usage And Synthesis
DescriptionHEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical PropertiesWhite crystalline powder
UsesNon-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
UsesBiological buffer.
UsesHEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
DefinitionChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General DescriptionHEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological ActivityHEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

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