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Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China
Brand Name: DLdevelop
Certification: ISO9001:2015, CE
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
Model Number: DL-AGER-Hu
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Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit

Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit

two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.
Traditional ELISA KitReady-to-Use ELISA KIT
Product name:Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Method:Sandwich
Synonyms:

RAGE; Receptor For Advanced Glycation Endproducts

Detection range:0.156-10ng/mL
Reactivity:AGER
Size:96T/48T
Quality guarantee period:for 12 months
Catalog number:DL-AGER-Hu (traditional)DLR-AGER-Hu (ready-to-use)
Assay length1-4.5Hours1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual
1. Overview
Other names:RAGE; Receptor For Advanced Glycation Endproducts
Function:Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Sequence:
MAAGTAVGAW VLVLSLWGAV VGAQNITARI GEPLVLKCKG APKKPPQRLE
WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ
AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY
PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG
DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA
PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS
CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LALALGILGG
LGTAALLIGV ILWQRRQRRG EERKAPENQE EEEERAELNQ SEEPEAGESS
TGGP
2. Features
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AGER in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
DETECTION RANGE
0.156-10ng/mL. The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL.
SENSITIVITY
The minimum detectable dose of AGER is typically less than 0.058ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of AGER.
No significant cross-reactivity or interference between AGER and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between AGER and all analogues, therefore, cross reactivity may still exist.
IMPORTANT NOTES
1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.
2. The final experimental results will be closely related to the validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available to obtain accurate results.
3. Kits from different batches may be a little different in detection range, sensitivity and color developing time. Please perform the experiment exactly according to the instruction manual included in your kit. Electronic ones on our website are for reference only.
4. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
5. Protect all reagents from strong light during storage and incubation. All bottle caps of reagents should be closed tightly to prevent evaporation of liquids and contamination by microorganisms.
6. There may be a foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
7. Incorrect procedures during reagent preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
8. Even the same experimenter may get different results from two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before the general assay for each batch is recommended.
9. Each kit has undergone several rigorous quality control tests. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipment. Intra-assay variance among kits from different batches could arise from the above factors as well.
10. Kits from different manufacturers with the same item might produce different results, since we have not compared our products with other manufacturers.
11. The standard in this kit, as well as the antigens used in antibody preparation are typically recombinant proteins. Differently expressed sequences, expression systems, and/or purification methods can be used in the preparation of recombinant proteins. There is also the possibility of differences in the screening technique of antibodies and antibody pairs in our kits. As a result, we cannot guarantee that our kit will be able to detect recombinant proteins produced by other companies. We do NOT recommend using our ELISA kits for the detection of other recombinant proteins.
12. Validity period: 12 months.
13. The instruction manual also works with the 48T kit, but all reagents in the 48T kit are reduced by half.

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

You can reference link of the kit as following
http://www.dldevelop.com/Research-reagent/dl-ager-hu.html
http://www.dldevelop.com/uploadfile/data/DL-AGER-Hu.pdf
http://www.dldevelop.com/Research-reagent/dl-ager-mu.html
http://www.dldevelop.com/uploadfile/data/DL-AGER-Mu.pdf
Introduction
ItemStandardTest
Description

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AGER in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Conform
IdentificationColorimetricPositive
CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120μLDetection Solution A 1×12mL
Detection Reagent B 1×120μLDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mL
Instruction manual 1Instruction manual 1
Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AGER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGER in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant AGER and the recovery rates were calculated by comparing the measured value to the expected amount of AGER in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)84-10092
EDTA plasma(n=5)83-9991
heparin plasma(n=5)85-10193
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGER and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)82-96%83-98%81-99%93-101%
EDTA plasma(n=5)88-101%86-95%90-102%80-93%
heparin plasma(n=5)80-91%82-90%95-104%79-95%




























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