English Name:Piperazine-n,n’-Bis(2-Ethanesulfonic Acid),
Description:White crystalline powder
Appropriate PH is obtained by mixing mild acid and its conjugate
Many biological responses happen in neutral condition (usually PH
so the buffering range is required to be in PH 6-8. In addition,
of acid-base of buffer should not chelate with some metallic ions.
Characteristics of biological buffer:
1. Strong water-solubility;
2. Compatibility of the acid-base dissociation balance;
3. Low membrance penetration rate;
4. Low metal chelating ability;
5. High chemical stability;
6. Low absorption in ultraviolet and visible region.
Application: it is an important PH stable reagent in biotic
It is frequently used as a buffering agent in biochemistry.
It is an ethanesulfonic acid buffer developed by Good et al. in the
1960s. PIPES has a pKa near the physiological pH which makes it
useful in cell culture work.
It has been documented to minimize lipid loss when buffering
glutaraldehyde histology in plant and animal tissues.
Additional forms available: PIPES, Free Acid ; PIPES, Sesquisodium
Salt; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium