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Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

Categories Recombinant Protein A
Brand Name: YaxinBio
Model Number: RSPA05
Certification: ISO 9001 2015
Place of Origin: China
MOQ: 10mg
Payment Terms: T/T
Packaging Details: ice packaging
Price: 79$/10mg
Supply Ability: 1000g per week
brand: yaxinbio
Substrate: Porous polystyrene-divinylbenzene
Ligand: Recombinant Protein A
Recommend flow rate: 300-700 cm/h
DBC: At least 300 mg IgG/mL media at 500 cm/h
Maximum pressure: 1500 PSI
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Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A

Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A


Protein A

CAS:91932-65-9

Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.


Application(1)


The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.


APPLICATION(2)


The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.


MAIN FEATURES

AppearanceWhite or off white powder
SolubilityClear liquid
Bioburden≤ 1 cfu/ mg
SDS-PAGESingle major band
SDS-PAGE (MW)25000±1000 Daltons
UV SpectrumE275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC)≥ 95%

STABILITY OF STORAGE AND TRANSPORT


Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

After dissolved with pure water, it should be stored under -20℃.


Stability of transport: The product is stable by blue ice insulation transport.


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Product Tags:

SPA protein A

  

purified protein

  
Quality Protein A Recombinant Protein A E.Poli Not Antibody Column Purified Protein A for sale
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